5-1．簡単な解説
消化薬「タカジアスターゼ」の生産菌（Aspergillus oryzae）の1株のタンパク質分解酵素を精製して性質を特定し,作用機構を解明した（原著論文1-8）。放線菌（Streptomyces sp.）の1株から新コラゲナーゼを発見し、性質と作用機構を特定した。本酵素はヤクルト薬品がガンなどの研究試薬として商業化（原著論文９）。
5-2. 原著論文(9編）
- 1) Tsujita Y, Endo A. Purification and characterization of the two molecular forms of Aspergillus oryzae acid protease. Biochim Biophys Acta 1976; 445: 197-204.
- 2) Tsujita Y, Endo A. Chemical properties of the polysaccharides associated with acid protease of Aspergillus oryzae grown on solid bran media. J Biochem 1977; 81: 1063-1070.
- 3) Tsujita Y, Endo A. Evidence for the presence of membrane-bound acid protease of Aspergillus oryzae. Biochem Biophys Res Commun 1977; 74: 242-247.
- 4) Tsujita Y, Endo A. Extracellular acid protease of Aspergillus oryzae grown on liquid media: multiple forms due to association with heterogeneous poly- saccharides. J Bacteriol 1977; 130: 48-56.
- 5) Tsujita Y, Endo A. Solubilization and activation of membrane-bound acid protease of Aspergillus oryzae. Agric Biol Chem 1977; 41: 2125-2130.
- 6) Tsujita Y, Endo A. Presence and partial characterization of internal acid protease of Aspergillus oryzae. Applied and Environmental Microbiol 1978; 36: 237-242.
- 7) Tsujita Y, Endo A. Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae. Eur J Biochem 1976; 84: 347-353.
- 8) Tsujita Y, Endo A. Intracelluar localization of two molecular forms of membrane acid protease in Aspergillus oryzae. J Biochem 1980; 88: 113-120.
- 9) Endo A, Murakawa S, Shimizu H, Shiraishi Y. Purification and properties of collagenase from a Streptomyces species. J Biochem 1987; 102: 163-170.
